App Note: How to Pipette PCR Master Mix for Increased Accuracy in qPCR Results

Pipetting technique can influence qPCR assay results. PCR Master mix is routinely used during qPCR set-up but can be challenging to pipette accurately. In this study, we determined the best pipetting technique for pipetting Master mix in qPCR assays. We tested forward and reverse pipetting techniques, the type of pipette tips, pre-wetting of the pipette tip, and the use of electronic pipettes and identified best practices for obtaining accurate qPCR results.

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